Comparison of results by four different procedures for determination of 17-hydroxycorticosteroids.

Indirect analysis of corticosteroids. I. The determination of 17-hydroxycorticosteroids.

Cefoxitin interferes with the "Clini-Skreen" column method for urinary 17-hydroxycorticosteroids.

Cefoxitin interferes with determination of urinary 17-hydroxycorticosteroids. The apparent concentration of hormone is increased from three- to 10-fold in samples from patients receiving cefoxitin when the Amberlite XAD-2 "Clini-Skreen" column is used. To determine the mechanism of interference, we reacted aqueous solutions of cefoxitin, cortisol, cortisone, and 11-deoxycortisol with phenylhydr...

The use of tetramethylammonium hydroxide in the Zimmermann reaction.

The use of tetramethylammonium hydroxide (T.M.A.H.) in place of potassium hydroxide in the Zimmermann reaction has been investigated. Although various pure steroids have different colour equivalents, a comparison of the results of 17-ketosteroid and 17-hydroxycorticosteroid estimations on a series of urines showed that the differences for the two reagents were only small and may be ignored. Tet...

A Modified Procedure for the Estimation of Total 17-hydroxycorticosteroids (porter-silber Chromogens) in Urine.

A modified procedure for the determination of urinary 17-hydroxycorticosteroids(dihydroxy-acetonyl-corticoids) is described. The use of ethyl ether in a single extraction step and the stabilization of the phenylhydrazine-sulfuric acid reagent with sodium chloride are the unique features of the method. Studies of chromogenicities of steroids, recovery, pH effect on extraction, and salt effect on...

Use of control sera for monitoring temperature changes during pH and pCO2 measurements.

hydrochloride (10 mg three times a day for as long as 20 days) did not affect values for 17-hydroxycorticosteroids. In vitro data do show that there is a small increase in the apparent value for urinary 17-hydroxycorticosteroids when chlordiazepoxide or its lactam or N-despiethyl metabolite is added to urine. However, this increase does not appear to be large enough to be of significance. The f...